grade 2 mutant idh2 sw1353 cell line Search Results


grade 2 mutant idh2 sw1353 cell line  (ATCC)
96
ATCC grade 2 mutant idh2 sw1353 cell line
Ugonin V inhibits chondrosarcoma cell migration and invasion in a concentration-dependent manner. (A) The chemical structure of ugonin V. (B) JJ012 and <t>SW1353</t> cells were treated with different concentrations of ugonin V (1 μM, 3 μM, and 10 μM) for 24 and 48 h, MTT assay was used to evaluate the cytotoxicity effect. (C&D) Migration and invasion assay determined the migration and invasion ability of JJ012 and SW1353 cells after 24 h incubated in different concentrations of ugonin V (1 μM, 3 μM, 10 μM). * p < 0.05.
Grade 2 Mutant Idh2 Sw1353 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Ugonin V inhibits chondrosarcoma cell migration and invasion in a concentration-dependent manner. (A) The chemical structure of ugonin V. (B) JJ012 and SW1353 cells were treated with different concentrations of ugonin V (1 μM, 3 μM, and 10 μM) for 24 and 48 h, MTT assay was used to evaluate the cytotoxicity effect. (C&D) Migration and invasion assay determined the migration and invasion ability of JJ012 and SW1353 cells after 24 h incubated in different concentrations of ugonin V (1 μM, 3 μM, 10 μM). * p < 0.05.

Journal: International Journal of Biological Sciences

Article Title: Ugonin inhibits chondrosarcoma metastasis through suppressing cathepsin V via promoting miR-4799-5p expression

doi: 10.7150/ijbs.106827

Figure Lengend Snippet: Ugonin V inhibits chondrosarcoma cell migration and invasion in a concentration-dependent manner. (A) The chemical structure of ugonin V. (B) JJ012 and SW1353 cells were treated with different concentrations of ugonin V (1 μM, 3 μM, and 10 μM) for 24 and 48 h, MTT assay was used to evaluate the cytotoxicity effect. (C&D) Migration and invasion assay determined the migration and invasion ability of JJ012 and SW1353 cells after 24 h incubated in different concentrations of ugonin V (1 μM, 3 μM, 10 μM). * p < 0.05.

Article Snippet: The grade 2 mutant IDH2 SW1353 cell line was purchased from ATCC (Manassas, VA, USA) and the grade 2 mutant IDH1 JJ012 cell line was a gift from Dr. Sean P. Scully (University of Miami School of Medicine in Miami, FL, USA).

Techniques: Migration, Concentration Assay, MTT Assay, Invasion Assay, Incubation

Over-expression of CTSV reverses ugonin V effect in chondrosarcoma cell migration and invasion. (A&B) Western blot analysis of CTSV expression in JJ012 and SW1353 after pcDNA3.1(+)/CTSV vector and empty vector were transfected. (C&D) Parental and CTSV-overexpressing JJ012 and SW1353 cell lines were treated with 10 μM ugonin V for 24 h, cell motility was respectively analyzed by migration and invasion assays. * p < 0.05 compared to control group; # p < 0.05 compared to the ugonin V-treated group.

Journal: International Journal of Biological Sciences

Article Title: Ugonin inhibits chondrosarcoma metastasis through suppressing cathepsin V via promoting miR-4799-5p expression

doi: 10.7150/ijbs.106827

Figure Lengend Snippet: Over-expression of CTSV reverses ugonin V effect in chondrosarcoma cell migration and invasion. (A&B) Western blot analysis of CTSV expression in JJ012 and SW1353 after pcDNA3.1(+)/CTSV vector and empty vector were transfected. (C&D) Parental and CTSV-overexpressing JJ012 and SW1353 cell lines were treated with 10 μM ugonin V for 24 h, cell motility was respectively analyzed by migration and invasion assays. * p < 0.05 compared to control group; # p < 0.05 compared to the ugonin V-treated group.

Article Snippet: The grade 2 mutant IDH2 SW1353 cell line was purchased from ATCC (Manassas, VA, USA) and the grade 2 mutant IDH1 JJ012 cell line was a gift from Dr. Sean P. Scully (University of Miami School of Medicine in Miami, FL, USA).

Techniques: Over Expression, Migration, Western Blot, Expressing, Plasmid Preparation, Transfection, Control

MiR-4799-5p directly binds to CTSV 3'-UTR to reduce CTSV synthesis and suppress chondrosarcoma cell migration and invasion. (A) The graphic illustrates the binding site of miR-4799-5p on CTSV 3'-UTR predicted by the TargetScan database. (B) Relative luciferase activities of chondrosarcoma cells transfected with a CTSV 3'-UTR luciferase reporter vector, after 24 h of ugonin V treatment. JJ012 and SW1353 cell lines were transfected with a miR-4799-5p inhibitor and negative control, followed by 24 h of ugonin V treatment (10 μM). (C-E) Western blotting and qPCR analysis were used to determine CTSV expression. (F&G) Chondrosarcoma cell motility was analyzed by migration and invasion assays. * p < 0.05 compared to control group; # p < 0.05 compared to the ugonin V-treated group. WT, wildtype; MUT, mutant; 3'-UTR, three prime untranslated regions; NC, negative control.

Journal: International Journal of Biological Sciences

Article Title: Ugonin inhibits chondrosarcoma metastasis through suppressing cathepsin V via promoting miR-4799-5p expression

doi: 10.7150/ijbs.106827

Figure Lengend Snippet: MiR-4799-5p directly binds to CTSV 3'-UTR to reduce CTSV synthesis and suppress chondrosarcoma cell migration and invasion. (A) The graphic illustrates the binding site of miR-4799-5p on CTSV 3'-UTR predicted by the TargetScan database. (B) Relative luciferase activities of chondrosarcoma cells transfected with a CTSV 3'-UTR luciferase reporter vector, after 24 h of ugonin V treatment. JJ012 and SW1353 cell lines were transfected with a miR-4799-5p inhibitor and negative control, followed by 24 h of ugonin V treatment (10 μM). (C-E) Western blotting and qPCR analysis were used to determine CTSV expression. (F&G) Chondrosarcoma cell motility was analyzed by migration and invasion assays. * p < 0.05 compared to control group; # p < 0.05 compared to the ugonin V-treated group. WT, wildtype; MUT, mutant; 3'-UTR, three prime untranslated regions; NC, negative control.

Article Snippet: The grade 2 mutant IDH2 SW1353 cell line was purchased from ATCC (Manassas, VA, USA) and the grade 2 mutant IDH1 JJ012 cell line was a gift from Dr. Sean P. Scully (University of Miami School of Medicine in Miami, FL, USA).

Techniques: Migration, Binding Assay, Luciferase, Transfection, Plasmid Preparation, Negative Control, Western Blot, Expressing, Control, Mutagenesis